U2 small nuclear RNA auxiliary factor 1
Splicing factor U2AF 35 kDa subunit is a protein that in humans is encoded by the U2AF1 gene.[1][2][3]
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.[3]
Interactions
U2 small nuclear RNA auxiliary factor 1 has been shown to interact with RP9,[4] U2AF2,[5][6] NXF1,[7] ASF/SF2[8][9] and ZRANB2.[10]
References
- ^ Lalioti MD, Gos A, Green MR, Rossier C, Morris MA, Antonarakis SE (Jan 1997). "The gene for human U2 snRNP auxiliary factor small 35-kDa subunit (U2AF1) maps to the progressive myoclonus epilepsy (EPM1) critical region on chromosome 21q22.3". Genomics 33 (2): 298–300. doi:10.1006/geno.1996.0196. PMID 8660980.
- ^ Kalcheva I, Plass C, Sait S, Eddy R, Shows T, Watkins-Chow D, Camper S, Shibata H et al. (Dec 1994). "Comparative mapping of the imprinted U2afbpL gene on mouse chromosome 11 and human chromosome 5". Cytogenet Cell Genet 68 (1–2): 19–24. doi:10.1159/000133881. PMID 7956352.
- ^ a b "Entrez Gene: U2AF1 U2 small nuclear RNA auxiliary factor 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7307.
- ^ Maita, Hiroshi; Kitaura Hirotake, Keen T Jeffrey, Inglehearn Chris F, Ariga Hiroyoshi, Iguchi-Ariga Sanae M M (Nov. 2004). "PAP-1, the mutated gene underlying the RP9 form of dominant retinitis pigmentosa, is a splicing factor". Exp. Cell Res. (United States) 300 (2): 283–296. doi:10.1016/j.yexcr.2004.07.029. ISSN 0014-4827. PMID 15474994.
- ^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct. 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514.
- ^ Zhang, M; Zamore P D, Carmo-Fonseca M, Lamond A I, Green M R (Sep. 1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 89 (18): 8769–8773. doi:10.1073/pnas.89.18.8769. ISSN 0027-8424. PMC 50002. PMID 1388271. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=50002.
- ^ Zolotukhin, Andrei S; Tan Wei, Bear Jenifer, Smulevitch Sergey, Felber Barbara K (Feb. 2002). "U2AF participates in the binding of TAP (NXF1) to mRNA". J. Biol. Chem. (United States) 277 (6): 3935–3942. doi:10.1074/jbc.M107598200. ISSN 0021-9258. PMID 11724776.
- ^ Xiao, S H; Manley J L (Nov. 1998). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2". EMBO J. (ENGLAND) 17 (21): 6359–6367. doi:10.1093/emboj/17.21.6359. ISSN 0261-4189. PMC 1170960. PMID 9799243. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170960.
- ^ Zhang, W J; Wu J Y (Oct. 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. (UNITED STATES) 16 (10): 5400–8. ISSN 0270-7306. PMC 231539. PMID 8816452. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=231539.
- ^ Adams, D J; van der Weyden L, Mayeda A, Stamm S, Morris B J, Rasko J E (Jul. 2001). "ZNF265--a novel spliceosomal protein able to induce alternative splicing". J. Cell Biol. (United States) 154 (1): 25–32. doi:10.1083/jcb.200010059. ISSN 0021-9525. PMC 2196870. PMID 11448987. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2196870.
Further reading
- Zhang M, Zamore PD, Carmo-Fonseca M et al. (1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–8773. doi:10.1073/pnas.89.18.8769. PMC 50002. PMID 1388271. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=50002.
- Zamore PD, Green MR (1991). "Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution". EMBO J. 10 (1): 207–14. PMC 452631. PMID 1824937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=452631.
- Wu JY, Maniatis T (1994). "Specific interactions between proteins implicated in splice site selection and regulated alternative splicing". Cell 75 (6): 1061–1070. doi:10.1016/0092-8674(93)90316-I. PMID 8261509.
- Zuo P, Maniatis T (1996). "The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing". Genes Dev. 10 (11): 1356–1368. doi:10.1101/gad.10.11.1356. PMID 8647433.
- Hong W, Bennett M, Xiao Y et al. (1997). "Association of U2 snRNP with the spliceosomal complex E". Nucleic Acids Res. 25 (2): 354–361. doi:10.1093/nar/25.2.354. PMC 146436. PMID 9016565. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=146436.
- Tronchère H, Wang J, Fu XD (1997). "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA". Nature 388 (6640): 397–400. doi:10.1038/41137. PMID 9237760.
- Wang HY, Lin W, Dyck JA et al. (1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–750. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2141757.
- Rudner DZ, Kanaar R, Breger KS, Rio DC (1998). "Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo". Mol. Cell. Biol. 18 (4): 1765–73. PMC 121406. PMID 9528748. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=121406.
- Lallena MJ, Martínez C, Valcárcel J, Correas I (1998). "Functional association of nuclear protein 4.1 with pre-mRNA splicing factors". J. Cell. Sci. 111 ( Pt 14): 1963–71. PMID 9645944.
- Gozani O, Potashkin J, Reed R (1998). "A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site". Mol. Cell. Biol. 18 (8): 4752–60. PMC 109061. PMID 9671485. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=109061.
- Neubauer G, King A, Rappsilber J et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
- Xiao SH, Manley JL (1999). "Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2". EMBO J. 17 (21): 6359–6367. doi:10.1093/emboj/17.21.6359. PMC 1170960. PMID 9799243. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170960.
- Hattori M, Fujiyama A, Taylor TD et al. (2000). "The DNA sequence of human chromosome 21". Nature 405 (6784): 311–319. doi:10.1038/35012518. PMID 10830953.
- de Vries H, Rüegsegger U, Hübner W et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–5904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305781.
- Gama-Carvalho M, Carvalho MP, Kehlenbach A et al. (2001). "Nucleocytoplasmic shuttling of heterodimeric splicing factor U2AF". J. Biol. Chem. 276 (16): 13104–13112. doi:10.1074/jbc.M008759200. PMID 11118443.
- Adams DJ, van der Weyden L, Mayeda A et al. (2001). "ZNF265--a novel spliceosomal protein able to induce alternative splicing". J. Cell Biol. 154 (1): 25–32. doi:10.1083/jcb.200010059. PMC 2196870. PMID 11448987. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2196870.
- Will CL, Schneider C, MacMillan AM et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–4546. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125580.
- Nesic D, Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. 21 (19): 6406–6417. doi:10.1128/MCB.21.19.6406-6417.2001. PMC 99788. PMID 11533230. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99788.
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PDB gallery
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1jmt: X-ray Structure of a Core U2AF65/U2AF35 Heterodimer
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